Liquid Culture
Lignin-Prime LC Broth
Lignin-Prime
Liquid Culture
Liquid CultureVALIDATED
Lignin-Prime LC Broth
$18.00
Lignin-Prime liquid culture at 250 mL RTU scale: 2g Maltoferm extract + 2g Dextrose + 0.4g Soy Peptone + 0.2g Yeast Extract + 0.1g Tannic Acid / Oak Extract + 0.125g KH2PO4 + 0.05g MgSO4 in distilled water. Tannic acid / oak extract fraction provides lignocellulosic priming signal for wood-loving species. Reduced sugar load versus Vigor protects sensitive medicinals from osmotic stress during initial expansion. Sterilized at 118.3°C / 15 PSI for 39 minutes (elevation-adjusted, 4,984 ft). Shipped with 30 mm PTFE stir bar, Manifest Card, and QR-linked Digital Birth Certificate.
STERILIZATION
118.3°C @ 15 PSI / 39 min (elevation-adjusted)
VOLUME
250 mL
FORMULATION
Maltoferm extract + Dextrose + Soy Peptone + Yeast Extract + Tannic Acid / Oak Extract + KH2PO4 + MgSO4
PER-JAR ACTIVES
2g Maltoferm extract + 2g Dextrose + 0.4g Soy Peptone + 0.2g Yeast Extract + 0.1g Tannic Acid / Oak Extract + 0.125g KH2PO4 + 0.05g MgSO4
PRIMER COMPONENT
Tannic Acid / Filtered Oak Sawdust Extract
CONTAINER
250 mL borosilicate glass, GL45 PP screw cap
STIR BAR
30 mm PTFE magnetic (included)
ALTITUDE
4,984 ft (Longmont, CO)
MIN VIABLE QA HOLD (78–82°F INCUBATION)
3 days
BATCH SIZE
14 units per batch
INTEGRATION
QR → Cynic Lab OS (auto-log batch metadata on scan)
Integration
This unit includes a QR-linked tag. Scan to auto-populate batch metadata into your portal dashboard — sterilization parameters, QA hold window, F₀ value, and operator sign-off.
Every Unit Includes
- ✓1× 250ml borosilicate glass specimen bottle with GL45 blue screw cap (sterile)
- ✓1× 30mm PTFE magnetic stir bar
- ✓1× Physical Manifest Card
- ✓1× pH test strip affixed to Manifest Card
- ✓Batch ID + QR code linking to Digital Birth Certificate
Available for local pickup in Longmont, CO. Contact us to place an order.
Species Compatibility
Tannic acid/oak extract fraction provides lignocellulosic signaling for wood-rotting species. Faster colonization initiation reported on Lion's Mane, Reishi, and Turkey Tail versus sugar-only broths. Shiitake/Maitake: lower sugar load prevents early hyphal stalling. Chaga: reduces false-start cycles versus generic LC media. Use when transferring to hardwood-based spawn or substrate.
Validation Timeline
PREP_DATE
Logged per batch
QA_HOLD_PERIOD
78–82°F incubation window
RELEASE_DATE
Post-hold, zero-growth confirmed
Every unit is held in incubation after sterilization. No unit ships until the hold window closes with zero contamination evidence. Dates are recorded on the Digital Birth Certificate.
Digital Birth Certificate
Every unit ships with a Manifest Card carrying a QR code. Scan to access the thermal integration log, F₀ value, sterilization parameters, and operator sign-off for your batch. Data is immutable once published.
Batch Evidence
Use your shipped Batch ID to open the certificate at /batch/[BATCH-ID].
Altitude Compensation — 4,984 ft
Sterilization parameters compensated for operating altitude. At 4,984 ft, 15 PSI yields 118.3°C — not 121°C. Hold window is product-specific, validated to per-material F₀ targets. Sea-level timing applied at elevation produces inadequate lethality.
Liquid media runs use shorter lethality windows than dense solids. Released only after QA incubation review.